EFFECT OF THE BENCH SCALE EXTRACTION CONDITIONS ON Pinus radiata BARK EXTRACT YIELD, ANTIOXIDANT PROPERTIES AND COMPOSITION

Valentina Ramos, Carlos Bocalandro, Sebastián Riquelme, Verónica Sanhueza, Estrella Aspé, Marlene Roeckel, Katherina Fernández

Abstract


Many pine bark extracts have antioxidant properties. This work examined bench scale extraction onthe yield, the composition and antioxidant properties of Pinus radiata. Acetone solvent (98.7% w/w at120 °C) yielded maximum phenols concentration, tannins concentration, anti-radical activity (DPPH)and reducing activity (FRAP), although its extraction yield was the lowest. When the organic solventin the mixture decreased, the chelating activity increased (ICA) and antiradical and reducing activitiesdecreased. The extract largest yield was obtained with water at 120ºC, but the antioxidant properties wererelatively poor. Taxifolin (dihydroquercetin) and catechin were the main extract components detectedby RP-HPLC. The best extraction conditions for high extractive yield and antioxidant properties wasachieved with ethanol/water at 120 °C.

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